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Тема/ВариантИзучение механизмов генерации АТФ - индуцированных внутриклеточных кальциевых сигналов в нервных клетках моторной коры
ПредметФизика
Тип работыкурсовая работа
Объем работы49
Дата поступления12.12.2012
890 ₽

Содержание

1. ВВЕДЕНИЕ 2. ОБЗОР ЛИТЕРАТУРЫ 2.1 Гомеостаз кальция в нервных клетках 2.1.1 Кальциевые каналы плазматической мембраны 2.1.2 Кальциевые буферы 2.1.3 Кальциевые каналы эндоплазматического ретикулума 2.1.4 Кальциевые насосы 2.1.5 Кальциевые обменники 2.1.6 Са2+-связывающие органеллы 2.2 Влияние АТФ на кальциевый гомеостаз

Введение

Молекула АТФ давно известна как повсеместно распространенный источник энергии для внутриклеточного метаболизма. Но ее свойства как нейротрансмитера были обнаружены сравнительно недавно. Сегодня уже не осталось никаких сомнений, что АТФ является нейротрансмитером в автономных нейромышечных соединениях, ганглиях и центральной нервной системе. К примеру, было показано, что АТФ вовлечена в генерацию болевых сигналов через Р2Х1 и Р2Х2 рецепторы. Однако роль и распределение пуринорецепторов в коре головного мозга и особенно в моторной коре до сих пор остается слабо изученной. Поэтому изучение механизмов действия АТФ в коре головного мозга представляет несомненный интерес. Мы изучали действие АТФ посредством измерения концентрации внутриклеточного кальция, - одного из найболее важных и универсальных регуляторов клеточных функций. Цель работы состояла в изучении механизмов генерации АТФ - индуцированных внутриклеточных кальциевых сигналов в нервных клетках моторной коры.

Литература

1. A.Shmigol, A.Verkhratsky & G. Isenberg (1995): Calcium-induced calcium release in rat sensory neurones. Journal of Physiology (London), 489.3 627-636. 2. A.Shmigol, G. Isenberg, P.Kostyuk & A. Verkhratsky (1994): Calcium-induced Ca2+ release from internal stores in rat dorsal root ganglion neurones. In: European Journal of Neuroscience, Suppl. 7, Proceedings of the 16th Annual ENA Meeting, p. 146. 3. A.Shmigol, N.Svichar, P.Kostyuk & A.Verkharatsky. (1995): "Incremental" caffeine-induced calcium release in mouse sensory neurones. European Joutnal of Neuroscience, Supple № 8. p111. Proceedings of the 16th Annual ENA Meeting. 4. A.Shmigol, Yu.Usachev, N.Pronchuk, S.Kirischuk, P.Kostyuk & A.Verkhratsky (1994): Properties of the caffeine sensitive intracellular calcium stores in mammalian neurons. Neurophysiology /Neirophiziologia, v. 26 No. 2, p. 16 - 25. 5. A.Verkhratsky, A. Shmigol, S. Kirischuk, N. Pronchuk & P. Kostyuk (1994): Age-dependent changes in calcium currents and calcium homeostasis in mammalian neurons. Annals of the New York Academy of Sciences, v. 747, p365 - 381. 6. Abbracchio, M. P., Burnstock, G. (1994) Purinoceptors: are there families of P2x and P 2y purinoceptors? Pharmac. Ther. 64: 445-475 7. Anatoly Smigol, Platon Kostyuk, Alexey Verhratsky (1994) Role of caffeine-sensitive Ca2+ stores in Ca2+ signal termination in adult DRG neurones // NeuroReport v.5, 2073-2076. 8. Anatoly Smigol, Sergey Kirischuk, Platon Kostyuk, Alexey Verhratsky (1994) Different properties of caffeine-sensitive Ca2+ stores in peripherial and central mammalian neurones // Pflugers Arch v.426, 174-176. 9. Baker P. F., Blaustein M.P., Hodgkin A.L. and Steinhardt R. A. (1969) The influence of calcium on sodium efflux in squid axons. J. Physiol., Lond. 200, 431 458. 10. Bean B.P. (1992) Pharmacology and electrophysiology of ATP activated ion channels. Trends Pharmacol. Sci. 13, 87 90. 11. Belan P., Kostyuk P., Snitsarev V. and Tepikin A. (1993) Calcium clamp in isolated neurones of the snail Helix pomatia. J. Physiol., Lond. 462, 47 58. 12. Bronner, F. (1990). Intracellular Ca2+ regulation.. New York: Wiley Liss. 13. Burk S. E., Lytton J. , MacLennan D. H. and Shull G. E. (1989). cDNA cloning, functional expressing, and mRNA tissue distribution of a third organellar Ca2+ pump. J. Biol. Chem. 164, 18561 18568. 14. Burnstock, G. (1972) Purinergic nerves. Pharmacol. Rev. 24: 509-581 15. Burnstock, G. (1978) A basis for distinguishing two types of purinergic receptor. in: book 16. Burnstock, G. (1990) Co-transmission. Arch. Int. Pharmacodyn. 304: 7-33 17. Burnstock, G., Kennedy, C.(1985) Is there a basis for distinguishing two types of P2 purinoceptor? Gen.Pharmacol. 16: 433-440 18. Carafoli E. (1992) Calcium pump of the plasma membrane. Physiol. Rev. 71, 129 153. 19. Chen, C.-C., Akopian, A.N. et al, (1995) A P2x purinoceptors expressed by a sybset of sensory neurones. Nature 377: 428 - 431 20. Кришталь О.А., Марченко С.М. (1983). Рецепторы АТФ в сенсорных нейронах млекопитающих. Докл. Акад. Наук УССР. 21. Gianini G., Clementi E., Ceci R., Marziali G., and Sorremtino V. (1992) Expression of a ryanodine receptor Ca2+ that is regulated by TGF b, Science, 257, 91 94. 22. Ginetta Collo et al, (1996) Cloning of P2X5 andP2X6 receptors and the distribution and properties of an extended family of ATP-gated ion channels. The J. of Neurosci. 16(8): 2495-2507 23. Gordon, J. L. (1986) Extracellular ATP: effects, sources and fate. Biochem.J. 233: 309-319 24. Grynkiewicz, G., Poenie, M., and Tsien, R.Y. A new generation of Ca2+ indicators with greatly improved fluorescent properties. J. Biol. Chem., 260, 3440-3450, 1985. 25. Heizmann C.W. and Hunziker W. (1991) Intracellular calcium binding proteins: more sights than insights. Trends Biochem. Sci. 16, 98 103. 26. Heschler J. and Schultz G. (1993) G proteins involved in the calcium channel signalling system. Curr. Opin. Neurobiol. 3, 360 367. 27. Hiderman, R. H., Martin, M., Zimmerman, J. K., Pivorun, E. B. (1991) Identification of a unique membrane receptor for adenosin 5 ,5 - P1,P4-tetraphosphate. J. Biol. Chem. 266: 6915-6918 28. Hoyle, C. H. V. (1990) Pharmacological activity of adenine dinucleotides in the periphery: possible receptor classes and transmitter function. Gen. Pharmacol. 21: 827-831 29. Hymel L., Inui M., Fleischer S. and Schindler H. (1988). Purified ryanodine receptor of skeletal muscle sarcoplasmic reticulum forms Ca2+ activated oligomeric Ca2+ channels in planar bilayers. Proc.Natl.Acad.Sci.U.S.A. 85, 441 445. 30. Kirischuk S.I., Voitenko N.V., Kettenmann H.O. and Verkhratsky A.N. (1994) Mechanisms of cytoplasmic calcium signalling in cerebellar Bergman glial cells // Neurophysiology v.26, 417-419. 31. Kirischuk, V.Matiash, A.Kulik, N.Voitenko, P.Kostyuk, A.Verkhratsky (1996) Activation of P2-purino, 1-adreno and H1-histamine receptors triggers cytoplasmic calcium signalling in cerebellar Pupkinje neurons // Neuroscience v.73, 643-647 32. Kostyuk and A. Verhratsky (1994) Calcium stores in neurones and glia //Neuroscience v. 63, N.2, 381-404. 33. Kostyuk P. G. (1992). Calcium ions in nerve cell function. Oxford, New York, Tokyo: Oxford University Press. 34. Kuno M., Maeda N. and Mikoshiba K. (1994) IP3 activated Ca2+ permeable channels in the incide out patches of cultured cerebellar Purkinje cells. Biochem. Biophys. Res. Comm. 199, 1128 1135. 35. Lуckhoff A. and Clapham D.E. (1992) Inositol 1,3,4,5 tetrakisphosphate activates an endothelial Ca2+ permeable channel. Nature 355, 356 358. 36. Londos, C., Cooper, D. M. F., Wolff, J. (1980) Subclasses of external adenosine receptors. Proc. Natl. Acad. Sci. 77: 2551-2554 37. Lytton J., Westlin M. and Hanley M. R. (1991). Thapsigargin inhibits the sarcoplasmic or endoplasmic reticulum Ca ATPase family of calcium pums. Biol. Chem. 266, 17067 17071. 38. Mackgrill J. J. and Lai F. A. (1994). Solubilization of the type 3 ryanodine receptor from rabbit brain. Biophys. J. 66, A147 39. McPherson P. S., Kim Y. K., Valdivia H., Knudson C. M., Takekura H., Franzini Armstrong C., Coronado R. and Campbell K. P. (1991). The brain ryanodine receptor: A caffeine sensitive calcium release channel. Neuron 7, 17 25. 40. N.Voitenko, S.Kirischuk, A.Kulik, A.Verkhratsky (1995) Calcium signalling in granule neurones of the mouse cerebellar slices // Pflugers Archiv European Journal of Physiology, v.430, Supplement 4, R124. 41. Nicholls D.G. (1985) A role for the mitochondria in the protection of the cell against calcium overload. Prog. Brain Res. 63, 97 106. 42. Pintor, J., Diaz-Rey, M. A., Torres, M., Miras-Portugal, M. T. (1992) Presence of diadenosine polyphosphates-Ap4A and Ap5A-in rat brain synaptic terminals. Ca2+-dependent release evoked by 4-aminopyridine and veratridine. Neurosci. Lett. 136: 141-144 43. Ribeiro, J. A., Sebastiao, A. M. (1986) Adenosine receptors and calcium: basis for proposing a third (A3) adenosine receptor. Prog. Neyrobiol. 26: 179-209 44. Rios E. and Pizarro C. (1991) Voltage sensor of excitation contraction coupling in skeletal muscle. Physiol. Rev. 76, 849 908 45. Ross C. A., Danoff S. K., Schell M. J., Snyder S. H. and Ullrich A. (1992). Three additional inositol 1,4,5 trisphosphate receptors: Molecular cloning and differential localization in brain and peripheral tissues. Proc. Natl. Acad. Sci. U.S.A. 89, 4265 4269. 46. S.Kirischuk, N.Voitenko, P.Kostyuk, A.Verkhratsky (1995) Calcium signalling in granule neurones studied in cerebellar slices // Cell Calcium v.18, 464-476 47. S.Kirischuk, N.Voitenko, P.Kostyuk, A.Verkhratsky (1996) Age-associated Changes of Citoplasmic Calcium Homeostasis in Cerebellar Granule Neurones in situ: Investigation on Thin Cerebellar Slices. // Experimental Gerontology 48. S.Kirischuk, N.Voitenko, T.Moller, H.Kettenmann and A.Verkhratsky (1995) ATP-induced cytoplasmic calcium mobilization in bergman glial cells // J. Neuroscience v.15, 8234-8248. 49. Scheggerburger R., Zhou Z., Konnerth A. and Neher E. (1993). Fractional contribution of calcium to the cation current through glutamate receptor channels. Neuron 11, 133 143. 50. Sergej Kirischuk and Alexej Verkhratsky (1996) [Ca2+]i recordings from neural cells in acutely isolated cerebellar slices employing differential loading of the membrane-permeant form of the calcium indicator fura-2 // Pflugers Arch. -Eur. J. Physiology v.431, 977-983 51. Sergej Kirischuk, Nana Voitenko, Platon Kostyuk, Alexej Verkhratsky (1996) Calcium signalling in granule neurones studied in cerebellar slices // Cell Calcium v.19, 59-71 52. Shmigol A., Kirischuk S., Kostyuk P. and Verkhratsky A. (1994). Different properties of caffeine sensitive Ca2+ stores in peripheral and central mammalian neurones. Pflьgers Arch. 426, 174 176. 53. Shmigol, D.Eisner & A.Verkhratsky (1995): Cyclic ADP ribose enhances Ca2+-induced Ca2+ release in mouse sensory neurones. Journal of Physiology, London, v. 483P, p63P. 54. Shmigol, N. Svichar, P. Kostyuk & A. Verkhratsky (1996) Gradual caffeine-induced Ca2+ release in mice DRG neurones is controlled by cytoplasmic and intraluminal Ca2+. Neuroscience, 73 N 4, 1061-1067 55. Shmigol, P. Kostyuk & A. Verkhratsky (1995): Thapsigargin blocks plasmalemmal voltage-operated calcium channels in mouse DRG neurones. Journal of Physiology, London, v. 483P, p64P. 56. Soltoff, S. P., McMillian, M.K., Talamo, B.R., Cantley, L. C.(1993) Blockade of ATP binding site of P2 purinoceptors in rat parotid acinar cells by isothiocyanate compounds. Biochem. Pharmacol. 45: 1936-1940 57. Tatsumi H. and Katayama Y. (1993) Regulation of intracellular free calcium concentration in acutely dissociated neurones from rat nucleus basalis.J.Physiol., Lond.464,165 181. 58. Tepikin A. V., Kostyuk P. G., Snitsarev V. A. and Belan P. V. (1992a). Extrusion of calcium from a single isolated neuron of the snail Helix pomatia. J. Membrane Biol. 123, 43 37. 59. Thayer S.A. and Miller R.J. (1990) Regulation of the intracellular free calcium concentration in single rat dorsal root ganglion neurones in vitro. J.Physiol. (London), 425, 85 115. 60. Ursula Windscheif, (1996) Purinoceptors: from history to recent progress. Review. J. Pharm. Pharmacol. 48: 993-1011 61. Van Calker, D., Muller, M., Hamprecht, B. (1979) Adenosine regulates via two different types of receptors, the accumulation of cyclic AMP in cultured brain cells. J. Neurochem. 33: 999-1005 62. Verkhratsky & A.Shmigol (1996) Calcium-induced calcium release in neurones. Cell Calcium, v.19, No 1, 1-14. 63. Voitenko N., Kirischuk S., Verkhratsky A. (1995) Mechanisms of cytoplasmic calcium signalling in cerebellar granule neurones in situ. // Експериментальна та клінічна фізіологія, збірник наукових праць до 100-річчя кафедри фізіології Львівського медичного університету, р.357. 64. Zhou Z. and Neher E. (1993). Calcium permeability of nicotininc acetylcholine receptor channels in bovine adrenal chromaffine cells. Pflugers Arch. 425, 511 517. 65. Zhou Z. and Neher E. (1993). Mobile and immobile calcium buffers in bovine adrenal chromaffin cells. J.Physiol., Lond. 469, 245 273.
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